VIVA GUIDE TO FIRST PROF (PART-1)- ANATOMY, BIOCHEM, PHYSIOLOGY...
siir shakeel is asking really easy stuffessential and non essential amino acidsstandard and non standard amino acidsallosteric enzymes and coperate bindingAcidosis and alkalosis, causes
henderson-haselbach equation, Pka ?anion gap
biochemical roles of vitamins
steroids and sterols mein differencebile salts
Sir asked me abt GAGs proteoglycans،glycoproteins n bile acid funtions!
external was tough because he asked stuff not really mentioned in te books.
bbut i can only tell what he asked me..
structres of protiens and bonds involved in it
supersecondary motifs and epitope
enzymes inhibitors as
External asked me abt immunoglobulins,their structure,diff between heavy n light chain structure,bence jones protein,how r they seprated 4m albumin n urine?proteins classification,keratin occurance n amino acid most abundant n it?,AG (albumin,Globulin)ratio,heat coagulation test.
Other question tyndall effect,coenzymes table,suicide inhibihion n allosteric inhibition graph curve with reason(distinction question),cobalamin absorption,pernicious anemia.
external asked me how heam is produced in body tell all the mechanism and enzymes,level of Na in body who it is regulated in body,diabetes insipidus,classification of carbohydrates and GAGS,
Starch n glycogen structural diff starch n cellulose structural diff,invert sugar with reason,sucrose structure bna k dekhao,what is hysteria,causes ov metabolic acidosis,chemical buffers n how r they written.
ROLE OF FOLIC ACID AND COENZYME IN WHICH IT IS PRESENTROLE OF COBALAMINE AND HOW WE MEASURE IT IN BODY?MYOGLOBIN AND HAEMOGLOBIN STRUCTURAL DIFFERENCEDOES MYOGLOBIN RELEASE OXYGEN AND AT WHICH CONCENTERATION OF OXYGENGLOBULAR PROTEIN AND AXIAL PROTEIN.WHICH TYPE IS PRESENT IN BODY?FORMULA TO FIND OUT WHETHER A PROTIN WILL BE GLOBULAR OR AXIAL?
Km & its significance
classification of enzymes & what is hydratase?
Co factor of carbonic anhydrase & Urease
GAG's , proteoglycan , glycoprotien
significance of SO4 groups in GAG's
How enzyme decrease energy of activation? Mechanism??
Diff. b/w Synthase & Synthetase
Effect of substrate conc. on rate of reaction
therapeutic uses of Enzymes? in myocardial infarction???
Gibbs Donnan equilibrium & its significance?
Kbi starch aur glycogen khaey hain?? forms??
Minerals,vitamins n enzymes are main start wale studnts jo batch mai hain un k liajo akhir mai hon un se sir carbohydrates n protein n myoglibin etc ziyada suna hain
Serum Calcium level? Clinical presentation of Calcium deficiency
sir shakel ny bht acha viva liya h unho ny mjh sy vitamins ki classificatin, co.enzymes, vitamin.A functions, night.blindness, beri.beri, visual.cycle draw krvaya, oxidative.phosphorylatins k componentsExternal.Names of water soluble vitamins, vitamn.B12 absorptin, functins, disease.
MAJOR VIVA QUESTIONS.EXTERNAL: all viva wos centred around proteins. started off wth zymogens their examples..? how is pepsinogen activated to pepsin? endopeptidases. examples? exopeptidases examples.? carboxy peptidases? wot are ligases, example.?electrophorosis se related questions.! GIT se proteolitic enzymes.
MAJOR VIVA QUESTIONS. INTERNAL: cytochrome p450, simple proteins, conjugated proteins( def +examples),lipoproteins defination examples. membrane proteins ki nature. LDL,VLDL, HDL, why are HDLs named so? chylomicrons, biotin, biocitin nd the reaction in which it is utilized, same questions with pento thenic acid nd thiiamine. dry beri beri(detail), wet beri beri,allosteric inhibition, niacin acts as coenzyme for?,co enzyme defination.? pyruvate dehydrogenase function, oxido reductases ki sub classifications,def nd 1 exampe each.! :(
Prctcl viva: milk residue n filtrate kaisay banta hai, acetic acid milk main daalne se kia cheez change hoti hai, ph controlling mechanisms in bodyMajor viva Internal: Metabolic alkalosis, Body main Ca ki forms, What is Tetany, Whyit occurs, level of ca in body, Pyridoxine detailed, tb k patients main pyridoxine kyun kam hoti hai , vit E ka name, action, Rancidity, name of Fatty acids,External: carbohydrates classification, which polysacchacaride occurs in humans, where is it located, where is it metabolized to form energy, fats ka energy production main role hai ya nae,How glycogen forms, enzyme acting on it, deficiency of which vitamins causes anemia, how does pyridoxine causes anemia, heme synthesis
internal= visual cycle why called a cyclic process.....MOST IMP DEFINE BIOCHMSTRY N ITS SIGNFCANCE,..:p,:P,,ENZYMES OF VISUL CYCLE..asked me ka enzyme ka nam lo i said carbonic anhydars he then asked its functn ...etc...then wht r ligases n relation btwenn ligases n lyase...anion gap signfcnce....retinoic acid.n its signfnce....
internal....FIGLU test, Shcilling test, wernickes korsakoff syndrome(in what people r community), selenecysteine, allosteric effect on enzymes and the reason for the sigmoid curve.
externa.....vit D(production,activation,metabolic role,deficincy rsults, osteomalacia, plasma findings...like alkaline phosphate etc)...whn does acid phosphatase appear in plasma...conjugated proteins...nucleoproteins.
why tetany develops in metabolic alkalosis even with normal Ca level,Ca k amount in body,xenobiotics....
extrnal askd...whch enzymes are increasad in myocardial infarction and their timing....essential fatty acids kahan use hotay hain..baqi q easy thay..
external = classification of fatty acids,iodine numbr,2 classes of complex lipids having ceramide as their base,lecithins,phospholipases & source of phospholipase A
External : Vit A maintenance of epithelium kesy krata haior Iron ki absorptionMucosal Block theoryserum Ca++ level
internal: polysaccharides, starch n cellulose k dfrnc, glucogen n starch me dfrnc, cardiolipin, glycerol kya hai, sphingosine kya hai, ceramide kya hai, cyclic amp, function, koi lipid jo k secondary messanger(diacylglycerole) oxidatv phosphorylation (def) substrate linked phosphoryltion
external: buffer, how buffers r formed, example, metabolic acidosis and caussof respiratry acidosis and compenation, alkali reserve.....
Sir shakeel: CerobrosidesVit B12, absorption, it's function.Pernicious anemia, why it is called so.Megaloblastic anemia, why it is called so.Physical Findings in them.Intrinsic factor, why it is not digested.Mylein degeneration k effects.
External: Synthesis of heme, it's regulatory enzyme.protoporphyrin ka structure. difference between uroporphyrinogen 1 and 3.Factors effecting heme synthesis.Vit b12, it's chemical name, it's coenzymes and their functions.Schiling's test.
External: Heme breakdown, porphyrias and their types. Mera to itna sa hi viva tha, iske baad usne farigh kar dia.External: Vitamers, Ascorbic Acid and its function in immunityInternal: Km and its significance, Basis of classification of proteins into alpha beta and gamma classes, Steroid and sterol difference, Function of Cholesterol and its forms in body, Bence Jones Proteins, Paraproteins, Selenocysteine, Chiral Carbon, D & L isomers, Dextrorotation and Levorotation, Oxidative Phosphorylation, Complexes, Why is NAD present in ETC and not NADP? Mutliple Myeloma.Heptproteins ,oncotic pressure,Milk alkali syndrome,difference btw coagulation and denaturation of proteins;example of ketoacids,colloid ,types of colloid,protective colloid,chemical test for vit.A